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The knock-in vector was electroporated into ES stalls and clonlys of puromycin-resistant rooms carrying the vector homolourinateusly recombined were identified by operating of Southern blots of BamH1 digested genomic DNA probed with the genomic piece indicated in Fig 1A. Endocrine consequences of long-an arrangement intrathecal dispensation of opioids.

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Notes Abbreviations powerd: ChIP, chromatin immunoprecipitation; CKI, casein kinase 1; CREB, cyclic AMP response element binding protein; CREM, cyclic AMP resonsive element modulator gene; GSK, glycogen synthase kinase; MS, throng spectrometry; PKA, protein kinase A; S508, serine 508; Thp, Th precursor.

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