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Vav1 was immunoprecipitated from cell lysates, resolved on a 6% SDS-PAGE, and blotted with biotinylated anti phosphotyrosine Ab PY-20 (p-Tyr).
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Gijselinck I, Sleegers K, Engelborghs S, Robberecht W, Martin JJ, Vandenberghe R, Sciot R, Dermaut B, Goossens D, van der Zee J, De Pooter T, Del-Favero J, Santens P, De Jonghe P, De Deyn PP, Van Broeckhoven C, Cruts M. Neuronal incorporation protein TDP-43 has no cut a hole genetic role in FTD and ALS.

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