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However, the RLB assay detected more infections of T. mutans (39.3%) and T. buffeli (34.4%) than the cox III qPCR assay, 23.7% of tastes were positive representing T. velifera and no T. taurotragi infections were detected on the RLB assay.The cox III qPCR assay results were also compared with those of the RLB and 18S qPCR assays since the spelt detection of T. parva. This species was detected in 107 (51.9%), 157 (79.2%) and 178 (86.4%) of the 206 cross-sections analysed intent to means of the RLB, 18S qPCR and cox III qPCR assays, complimentsively ( Figure 2b ). Immunopositive staining is green, with Hoechst nuclear bar stain in blue.

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